ANTI AGING BIOMEDICINE

There are people who reach the age of 85 in a very good physical and mental condition. There are others who have extensive cognitive difficulties and physical disorders already by the age of 60. This is why it is logical to think that a person’s biological age is more indicative of their health than their chronological age. If an anti-aging theory or program is developed, it has to be tested whether it works. In the laboratory, using experimental animals, it is relatively easy to determine whether a certain anti-aging regime extends the life span. Not so with humans, because a lifelong study seems at present far from possible – nobody wants to engage in dedicating their life to studying something for over 50 years without any knowledge if the results would be favorable. This is why there is a need to identify the effects this certain program has not only in the body’s systems, but in the general aging process. To determine a person’s biological age and to assess the effects of different anti-aging techniques scientist use the so-called biomarkers of aging. It is generally believed that seven major health areas are affected by aging: cardiovascular health, glucose regulation, brain function, muscle and skeletal health, endocrine function, immune system and oxidative stress. Biomarkers of aging are physical properties in the human body which indicate that the body is aging. It is indicators of the normal phenomena of growing old. They are not, however, simply things which change with age. In order to be called a biomarker, a factor has to satisfy a number of criteria. The best markers will be the ones which are not susceptible to influence from the outside environment. For example, in the US cholesterol levels increase with age, but this is due to the nature of the American diet and is not characteristic for other parts of the world. Thus, a true biomarker would satisfy the following criteria: A. The marker must predict the rate of aging and be a better predictor of life span than chronological age. B. It must be able to be tested on a regular basis C. It must work both for humans and other species, such as laboratory animals D. There is support from human clinical assessment and complementary research studies. E. The studies are based on a significant representative sample. F. The result is a clear association with aging. G. A relatively narrow standard deviation is present. So far, around 24 factors have met the criteria and can be considered biomarkers. They may be indicated especially for males or for females, and figures may vary between the sexes. Here is their list: 1. 17-ketosteroid/ 17-hydroxycortiosteroid ratio (male) 13. Handgrip strength 2. Ascorbic acid 14. Hemoglobin A1C 3. Basal Metabolic Rate 15. Lung capacity- FEV1 4. Blood pressure- pulse 16. Lung capacity- FVC 5. Blood pressure- systolic 17. Maximum oxygen update (male) 6. Body Mass Index (female) 18. Near vision 7. Caries index 19. Noradrenaline- plasma (male) 8. Creatinine clearance 20. Peridontal index 9. DHEA-S 21. PSA total (male) 10. Fibrinogen 22. Skin elasticity 11. Hair baldness (male) 23. Testosterone free (male) 12. Hair grayness 24. Zinc- serum In addition, there are also a number of other factors which may be considered partially biomarkers of aging. The main problem with these is that their reliability has not been confirmed through a sufficient amount of clinical and experimental data. These include body flexibility, blood urea nitrogen, LDL cholesterol, melatonin levels, static balance, serotonin levels and many others. They are to a certain degree indicative of a person’s biological age, but should not be confused with other general health factors, which do not have a clear association with age. Biomarkers of aging could be divided in three major categories. There are the ones which determine the biological age, e.g. skin elasticity and visual accommodation. There are markers which predict the remaining life expectancy; they include DHEA-S, hand grip strength, etc. Finally, there are factors which determine disease susceptibility, such as systolic blood pressure and glucose-tolerance tests. All of the biomarker tests can be classified either as laboratory tests (e.g. blood and urine tests) or as physical tests undertaken in a clinic.

J Steroid Biochem Mol Biol. 2003 Jun;85(2-5):329-35. Neuroactive steroids: new biomarkers of cognitive aging. Vallee M, Purdy RH, Mayo W, Koob GF, Le Moal M. INSERM U588, Institut F. Magendie, 1 rue Camille Saint-Saens, 33077 Bordeaux Cedex, France. Intensive studies in animals established that neuroactive steroids display neuronal actions and influence behavioral functions. We describe here investigations on the role of neuroactive steroids in learning and memory processes during aging and suggest their role as biomarkers of cognitive aging. Our work demonstrated the role of the steroid pregnenolone (PREG) sulfate as a factor underlying an individual's age-related cognitive decline in animals. As new perspectives of research we argue that knowing whether neuroactive steroids exist as endogenous neuromodulators and modulate physiologically behavioral functions is essential. To this end, a new approach using the sensitive, specific, and accurate quantitative determination of neuroactive steroids by mass spectrometry seems to have potential for examining the role of each steroid in discrete brain areas in learning and memory alterations, as observed during aging.